In this study, silver nanomaterials were synthesized in an environmentally friendly, rapid and simple way with the extract prepared using walnut leaves. Characterization of these materials was determined by UV-visiblespectrophotometer (UV-Vis.), Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction Diffractometer (XRD), Scanning Electron Microscopy (SEM), and Zeta potential analysis data. It was determined that silver nanomaterials have a maximum absorbance at 454.01 nm wavelength, 23.66 nm crystal nano size, spherical appearance and -11.53 mV zeta potential. For biomedical applications, anti-microbial effects were studied on pathogenic species. The Minimum Inhibition Concentrations (MIC) of these species were determined as 0.25-1.0 mg / L by microdilution method.

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